Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4192595..4193171 Replicon chromosome
Accession NZ_CP116085
Organism Escherichia coli strain DETEC-S566

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIC95_RS20805 Protein ID WP_001297643.1
Coordinates 4192884..4193171 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag PIC95_RS20800 Protein ID WP_000063156.1
Coordinates 4192595..4192897 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC95_RS20785 (4189191) 4189191..4191341 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PIC95_RS20790 (4191391) 4191391..4191594 + 204 WP_000467859.1 YbdD/YjiX family protein -
PIC95_RS20795 (4191605) 4191605..4192561 + 957 WP_001297640.1 GTPase -
PIC95_RS20800 (4192595) 4192595..4192897 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
PIC95_RS20805 (4192884) 4192884..4193171 - 288 WP_001297643.1 BrnT family toxin Toxin
PIC95_RS20810 (4193370) 4193370..4194067 - 698 Protein_4074 IS1 family transposase -
PIC95_RS20815 (4194124) 4194124..4194219 + 96 Protein_4075 dienelactone hydrolase family protein -
PIC95_RS20820 (4194365) 4194365..4195597 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
PIC95_RS20825 (4195638) 4195638..4196918 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4193370..4193579 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T267860 WP_001297643.1 NZ_CP116085:c4193171-4192884 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT267860 WP_000063156.1 NZ_CP116085:c4192897-4192595 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References