Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4487306..4487803 Replicon chromosome
Accession NZ_CP116074
Organism Escherichia coli strain DETEC-S586

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC49_RS21645 Protein ID WP_001173343.1
Coordinates 4487306..4487479 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC49_RS21650 Protein ID WP_000398619.1
Coordinates 4487507..4487803 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC49_RS21635 (4483977) 4483977..4485434 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
PIC49_RS21640 (4485671) 4485671..4487188 + 1518 WP_000003806.1 lysine--tRNA ligase -
PIC49_RS21645 (4487306) 4487306..4487479 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC49_RS21650 (4487507) 4487507..4487803 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC49_RS21655 (4488032) 4488032..4488304 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PIC49_RS21660 (4488316) 4488316..4488546 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
PIC49_RS21665 (4488727) 4488727..4490358 + 1632 WP_001216477.1 sensor histidine kinase -
PIC49_RS21670 (4490355) 4490355..4491074 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T267829 WP_001173343.1 NZ_CP116074:c4487479-4487306 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT267829 WP_000398619.1 NZ_CP116074:c4487803-4487507 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References