Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2946511..2947295 Replicon chromosome
Accession NZ_CP116074
Organism Escherichia coli strain DETEC-S586

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag PIC49_RS14285 Protein ID WP_000613626.1
Coordinates 2946801..2947295 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag PIC49_RS14280 Protein ID WP_001110447.1
Coordinates 2946511..2946804 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC49_RS14270 (2941661) 2941661..2942620 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
PIC49_RS14275 (2943193) 2943193..2946378 + 3186 WP_000827427.1 ribonuclease E -
PIC49_RS14280 (2946511) 2946511..2946804 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
PIC49_RS14285 (2946801) 2946801..2947295 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
PIC49_RS14290 (2947390) 2947390..2948343 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
PIC49_RS14295 (2948355) 2948355..2949998 - 1644 WP_000096524.1 flagellar hook-associated protein FlgK -
PIC49_RS14300 (2950064) 2950064..2951005 - 942 WP_001309406.1 flagellar assembly peptidoglycan hydrolase FlgJ -
PIC49_RS14305 (2951005) 2951005..2952102 - 1098 WP_000589320.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T267824 WP_000613626.1 NZ_CP116074:2946801-2947295 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT267824 WP_001110447.1 NZ_CP116074:2946511-2946804 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References