Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4665257..4665754 Replicon chromosome
Accession NZ_CP116071
Organism Escherichia coli strain DETEC-S589

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC30_RS23130 Protein ID WP_001173343.1
Coordinates 4665257..4665430 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag PIC30_RS23135 Protein ID WP_001312331.1
Coordinates 4665458..4665754 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC30_RS23120 (4661927) 4661927..4663384 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
PIC30_RS23125 (4663621) 4663621..4665138 + 1518 WP_000003806.1 lysine--tRNA ligase -
PIC30_RS23130 (4665257) 4665257..4665430 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC30_RS23135 (4665458) 4665458..4665754 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC30_RS23140 (4665983) 4665983..4666255 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PIC30_RS23145 (4666267) 4666267..4666497 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
PIC30_RS23150 (4666678) 4666678..4668309 + 1632 WP_001216482.1 sensor histidine kinase -
PIC30_RS23155 (4668306) 4668306..4669025 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T267798 WP_001173343.1 NZ_CP116071:c4665430-4665257 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT267798 WP_001312331.1 NZ_CP116071:c4665754-4665458 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References