Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1886996..1887827 Replicon chromosome
Accession NZ_CP116071
Organism Escherichia coli strain DETEC-S589

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag PIC30_RS09125 Protein ID WP_000854814.1
Coordinates 1886996..1887370 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag PIC30_RS09130 Protein ID WP_001285586.1
Coordinates 1887459..1887827 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC30_RS09085 (1882394) 1882394..1883559 + 1166 Protein_1779 serine-type D-Ala-D-Ala carboxypeptidase DacD -
PIC30_RS09090 (1883677) 1883677..1884150 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
PIC30_RS09095 (1884348) 1884348..1885406 + 1059 WP_001200890.1 FUSC family protein -
PIC30_RS09100 (1885578) 1885578..1885907 + 330 WP_000450409.1 DUF496 family protein -
PIC30_RS09105 (1886008) 1886008..1886331 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
PIC30_RS09110 (1886310) 1886310..1886390 + 81 WP_023441679.1 hypothetical protein -
PIC30_RS09115 (1886679) 1886679..1886759 - 81 Protein_1785 hypothetical protein -
PIC30_RS09120 (1886805) 1886805..1886999 - 195 WP_000988601.1 DUF5983 family protein -
PIC30_RS09125 (1886996) 1886996..1887370 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
PIC30_RS09130 (1887459) 1887459..1887827 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
PIC30_RS09135 (1887901) 1887901..1888122 - 222 WP_000692323.1 DUF987 domain-containing protein -
PIC30_RS09140 (1888191) 1888191..1888667 - 477 WP_001351157.1 RadC family protein -
PIC30_RS09145 (1888683) 1888683..1889156 - 474 WP_000855074.1 antirestriction protein -
PIC30_RS09150 (1889419) 1889419..1890240 - 822 WP_001234570.1 DUF932 domain-containing protein -
PIC30_RS09155 (1890461) 1890461..1890871 - 411 WP_000846704.1 hypothetical protein -
PIC30_RS09160 (1890887) 1890887..1891564 - 678 WP_001362823.1 hypothetical protein -
PIC30_RS09165 (1891700) 1891700..1892770 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T267780 WP_000854814.1 NZ_CP116071:c1887370-1886996 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T267780 NZ_CP116071:c1887370-1886996 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT267780 WP_001285586.1 NZ_CP116071:c1887827-1887459 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT267780 NZ_CP116071:c1887827-1887459 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References