Detailed information of TA system TA026777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 26687..27330
• Replicon: chromosome
• Accession: NC_017445
• Organism: Erwinia sp. Ejp617

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: E3DAW2
• Locus tag: EJP617_RS00125
• Protein ID: WP_014541899.1
• Coordinates: 26914..27330 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: E3DAW1
• Locus tag: EJP617_RS00120
• Protein ID: WP_014541898.1
• Coordinates: 26687..26917 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP617_RS00095	21931..22629	+	699	WP_014541895.1	RluA family pseudouridine synthase	-
EJP617_RS00100	22730..23269	-	540	WP_014541896.1	DUF2058 domain-containing protein	-
EJP617_RS00105	23457..25745	-	2289	WP_014541897.1	AAA family ATPase	-
EJP617_RS00120	26687..26917	+	231	WP_014541898.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EJP617_RS00125	26914..27330	+	417	WP_014541899.1	PIN domain-containing protein	Toxin
EJP617_RS00130	27557..28096	+	540	WP_041474217.1	hypothetical protein	-
EJP617_RS00135	28326..29207	+	882	WP_014541900.1	transposase	-
EJP617_RS00140	29358..30575	+	1218	WP_014541901.1	MFS transporter	-
EJP617_RS19205	30684..31336	-	653	Protein_26	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	31034..31336	302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15052.41 Da
• Isoelectric Point: 7.2442

>T26777 WP_014541899.1 NC_017445:26914-27330 [Erwinia sp. Ejp617]
MIKTYMLDTNICSFIMREQPEAVLNRLEQAVLRNHRVVVSAITYAEMRFGAIGKKASPRHAELVEAFCTRLDAVLAWDRL
AVDATTEIKAALAAAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLTLEYWAK

Nucleotide

• Length: 417 bp

>T26777 NC_017445:26914-27330 [Erwinia sp. Ejp617]
ATGATCAAGACCTACATGCTGGATACGAACATCTGTTCATTTATCATGCGTGAACAGCCGGAAGCGGTTCTGAACCGACT
GGAGCAGGCGGTGCTGCGCAATCACCGGGTCGTGGTGTCGGCCATCACCTACGCCGAGATGCGCTTCGGTGCGATCGGCA
AAAAAGCATCGCCCCGTCACGCAGAGCTGGTTGAAGCGTTCTGCACCCGTCTTGATGCGGTGCTTGCCTGGGACAGATTG
GCCGTGGATGCAACCACGGAAATCAAGGCGGCGCTGGCCGCTGCCGGCACGCCCATCGGCCCTAACGATACGGCAATCGC
CGGACACGCCATTGCTGCCGGGGCGGTTCTGGTGACGAACAATACGCGGGAGTTTGAGCGGGTGCCGGGGCTGACTCTGG
AATACTGGGCAAAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8712.74 Da
• Isoelectric Point: 4.6182

>AT26777 WP_014541898.1 NC_017445:26687-26917 [Erwinia sp. Ejp617]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWRSFSQLESADADFMAERGDVVGDEGRFDR

Nucleotide

• Length: 231 bp

>AT26777 NC_017445:26687-26917 [Erwinia sp. Ejp617]
ATGCGAACCGTATCCATATTTAAAAACGGCAACAACCGCGCCATCCGCCTGCCGCGCGATCTGGACTTTGAGGGCGTAAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTTCGCCCCGTCCGGCCCACCTGGCGCTCGTTCAGCCAGCTGG
AAAGTGCCGACGCGGACTTTATGGCGGAGCGCGGAGACGTTGTCGGCGATGAAGGAAGATTCGACCGATGA

Structures

Toxin

• AlphaFold DB: E3DAW2

Antitoxin

• AlphaFold DB: E3DAW1