Detailed information of TA system TA026776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 20095..20626
• Replicon: plasmid pJE01
• Accession: NC_017442
• Organism: Erwinia sp. Ejp617

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: E3DL65
• Locus tag: EJP617_RS17940
• Protein ID: WP_011078058.1
• Coordinates: 20095..20382 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: Q7X3U3
• Locus tag: EJP617_RS17945
• Protein ID: WP_011078059.1
• Coordinates: 20372..20626 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP617_RS17920	16595..18151	-	1557	WP_011113998.1	MCP four helix bundle domain-containing protein	-
EJP617_RS17925	18659..19072	-	414	WP_011113999.1	helix-turn-helix domain-containing protein	-
EJP617_RS17930	19073..19387	-	315	WP_041473956.1	type II toxin-antitoxin system HigB family toxin	-
EJP617_RS17935	19651..19935	-	285	WP_011114000.1	VF530 family protein	-
EJP617_RS17940	20095..20382	-	288	WP_011078058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP617_RS17945	20372..20626	-	255	WP_011078059.1	plasmid stabilization protein	Antitoxin
EJP617_RS17950	20836..21801	-	966	WP_011114001.1	ThiF family adenylyltransferase	-
EJP617_RS17955	21817..22575	-	759	WP_041474749.1	thiazole synthase	-
EJP617_RS17960	22579..22776	-	198	WP_014541855.1	sulfur carrier protein ThiS	-
EJP617_RS17965	22773..23705	-	933	WP_014541856.1	FAD-dependent oxidoreductase	-
EJP617_RS19170	24081..24272	-	192	Protein_25	Txe/YoeB family addiction module toxin	-
EJP617_RS17970	24422..25006	+	585	WP_011114004.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..30866	30866
	-	flank	IS/Tn	-	-	24422..25006	584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11197.09 Da
• Isoelectric Point: 10.6516

>T26776 WP_011078058.1 NC_017442:c20382-20095 [Erwinia sp. Ejp617]
MTYKLKFVPSAEKEWKRLGHPVREQFKKKLAERLENPRVPSAQLHGRKDQYKIKLKSAGYRLVYLVQEHTITIMVMGIGK
REGNQIYSDTENRLP

Nucleotide

• Length: 288 bp

>T26776 NC_017442:c20382-20095 [Erwinia sp. Ejp617]
ATGACTTATAAACTCAAGTTTGTCCCTTCCGCAGAGAAGGAATGGAAAAGGCTCGGGCACCCTGTCAGGGAGCAGTTCAA
AAAAAAGCTTGCTGAACGCCTGGAAAACCCCCGAGTGCCTTCAGCACAGCTTCATGGTCGTAAAGATCAGTACAAAATTA
AGCTAAAGTCTGCCGGTTACAGGCTGGTTTATCTGGTCCAGGAGCACACCATTACGATAATGGTTATGGGCATTGGCAAA
CGTGAAGGTAATCAGATTTACTCTGATACAGAGAACCGGCTTCCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9210.56 Da
• Isoelectric Point: 3.8705

>AT26776 WP_011078059.1 NC_017442:c20626-20372 [Erwinia sp. Ejp617]
MAFNILTNTAASITDLKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAMYAYLMELAEDAELGCIVDDRMSELPVAKEIS
LDDL

Nucleotide

• Length: 255 bp

>AT26776 NC_017442:c20626-20372 [Erwinia sp. Ejp617]
ATGGCATTCAACATACTCACTAATACCGCAGCAAGCATCACGGATTTGAAGCGCGACCCAATGGGAACAGTCCGTGCTGC
CGAAGGGGAAACTATCGCAATTTTAAACCGTAACGAGCCGGCATTTTATTGCGTACCACCCGCTATGTACGCTTATCTGA
TGGAGCTGGCAGAGGATGCTGAGCTTGGATGTATCGTTGACGATCGCATGTCAGAGTTACCGGTTGCGAAAGAGATATCA
TTGGATGACTTATAA

Structures

Toxin

• AlphaFold DB: E3DL65

Antitoxin

• AlphaFold DB: Q7X3U3