Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4194536..4195154 Replicon chromosome
Accession NZ_CP116046
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag PIH14_RS20315 Protein ID WP_001291435.1
Coordinates 4194536..4194754 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag PIH14_RS20320 Protein ID WP_000344800.1
Coordinates 4194780..4195154 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS20280 (4189825) 4189825..4190397 + 573 WP_000779831.1 YbaY family lipoprotein -
PIH14_RS20285 (4190428) 4190428..4190739 - 312 WP_000409911.1 MGMT family protein -
PIH14_RS20295 (4191118) 4191118..4191471 + 354 WP_000878140.1 DUF1428 domain-containing protein -
PIH14_RS20300 (4191513) 4191513..4193063 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
PIH14_RS20305 (4193227) 4193227..4193697 - 471 WP_000136192.1 YlaC family protein -
PIH14_RS20310 (4193813) 4193813..4194364 - 552 WP_000102564.1 maltose O-acetyltransferase -
PIH14_RS20315 (4194536) 4194536..4194754 - 219 WP_001291435.1 HHA domain-containing protein Toxin
PIH14_RS20320 (4194780) 4194780..4195154 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
PIH14_RS20325 (4195700) 4195700..4198849 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
PIH14_RS20330 (4198872) 4198872..4200065 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T267742 WP_001291435.1 NZ_CP116046:c4194754-4194536 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT267742 WP_000344800.1 NZ_CP116046:c4195154-4194780 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References