Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3681267..3681843 Replicon chromosome
Accession NZ_CP116046
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIH14_RS17810 Protein ID WP_001297643.1
Coordinates 3681267..3681554 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag PIH14_RS17815 Protein ID WP_000063160.1
Coordinates 3681541..3681843 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS17795 (3677505) 3677505..3678785 - 1281 WP_001037390.1 DUF445 domain-containing protein -
PIH14_RS17800 (3678826) 3678826..3680058 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
PIH14_RS17805 (3680372) 3680372..3681068 + 697 Protein_3471 IS1 family transposase -
PIH14_RS17810 (3681267) 3681267..3681554 + 288 WP_001297643.1 BrnT family toxin Toxin
PIH14_RS17815 (3681541) 3681541..3681843 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
PIH14_RS17820 (3681877) 3681877..3682833 - 957 WP_001297640.1 GTPase -
PIH14_RS17825 (3682844) 3682844..3683047 - 204 WP_000467859.1 YbdD/YjiX family protein -
PIH14_RS17830 (3683097) 3683097..3685247 - 2151 WP_271293096.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3680859..3681068 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T267739 WP_001297643.1 NZ_CP116046:3681267-3681554 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT267739 WP_000063160.1 NZ_CP116046:3681541-3681843 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References