Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3411624..3412121 Replicon chromosome
Accession NZ_CP116046
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIH14_RS16470 Protein ID WP_001173343.1
Coordinates 3411948..3412121 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIH14_RS16465 Protein ID WP_000398619.1
Coordinates 3411624..3411920 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS16445 (3408355) 3408355..3409074 - 720 WP_000611288.1 two-component system response regulator DcuR -
PIH14_RS16450 (3409071) 3409071..3410702 - 1632 WP_001216477.1 sensor histidine kinase -
PIH14_RS16455 (3410883) 3410883..3411113 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PIH14_RS16460 (3411125) 3411125..3411397 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
PIH14_RS16465 (3411624) 3411624..3411920 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIH14_RS16470 (3411948) 3411948..3412121 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIH14_RS16475 (3412240) 3412240..3413757 - 1518 WP_001295074.1 lysine--tRNA ligase -
PIH14_RS16480 (3413994) 3413994..3415451 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T267737 WP_001173343.1 NZ_CP116046:3411948-3412121 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT267737 WP_000398619.1 NZ_CP116046:3411624-3411920 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References