Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2023487..2024141 Replicon chromosome
Accession NZ_CP116046
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag PIH14_RS09825 Protein ID WP_000244777.1
Coordinates 2023487..2023894 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PIH14_RS09830 Protein ID WP_000354046.1
Coordinates 2023875..2024141 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS09805 (2019444) 2019444..2021177 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
PIH14_RS09810 (2021183) 2021183..2021893 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PIH14_RS09815 (2021918) 2021918..2022814 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PIH14_RS09820 (2022926) 2022926..2023447 + 522 WP_001055874.1 flavodoxin FldB -
PIH14_RS09825 (2023487) 2023487..2023894 - 408 WP_000244777.1 protein YgfX Toxin
PIH14_RS09830 (2023875) 2023875..2024141 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PIH14_RS09835 (2024384) 2024384..2025364 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
PIH14_RS09840 (2025560) 2025560..2026219 - 660 WP_000250274.1 hemolysin III family protein -
PIH14_RS09845 (2026383) 2026383..2026694 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
PIH14_RS09850 (2026739) 2026739..2028172 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
PIH14_RS09855 (2028229) 2028229..2028972 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T267733 WP_000244777.1 NZ_CP116046:c2023894-2023487 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT267733 WP_000354046.1 NZ_CP116046:c2024141-2023875 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References