Detailed information of TA system TA026773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 786437..786972
• Replicon: chromosome
• Accession: NC_017384
• Organism: Ketogulonicigenium vulgare WSH-001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KVU_RS03955
• Protein ID: WP_013384044.1
• Coordinates: 786724..786972 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: F9Y4N2
• Locus tag: KVU_RS03950
• Protein ID: WP_013384043.1
• Coordinates: 786437..786727 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVU_RS03910	781574..781978	+	405	WP_013384036.1	hypothetical protein	-
KVU_RS03915	781975..783129	+	1155	WP_013384037.1	hypothetical protein	-
KVU_RS03920	783129..783392	+	264	WP_013384038.1	hypothetical protein	-
KVU_RS03925	783505..784134	+	630	WP_044008023.1	TetR/AcrR family transcriptional regulator	-
KVU_RS03930	784134..784469	+	336	WP_014537641.1	DoxX family protein	-
KVU_RS03935	784617..784880	+	264	WP_013384040.1	hypothetical protein	-
KVU_RS03940	784880..786183	+	1304	Protein_779	hypothetical protein	-
KVU_RS03945	786198..786425	+	228	WP_013384042.1	hypothetical protein	-
KVU_RS03950	786437..786727	-	291	WP_013384043.1	BrnA antitoxin family protein	Antitoxin
KVU_RS03955	786724..786972	-	249	WP_013384044.1	BrnT family toxin	Toxin
KVU_RS03960	787069..787611	+	543	WP_013384045.1	lysozyme	-
KVU_RS03965	787615..787836	+	222	WP_013384046.1	hypothetical protein	-
KVU_RS03970	787836..788051	+	216	WP_162467560.1	hypothetical protein	-
KVU_RS03980	788242..788700	-	459	WP_014537644.1	sigma-70 family RNA polymerase sigma factor	-
KVU_RS03990	789845..790771	-	927	WP_014537645.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	747494..787611	40117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9028.54 Da
• Isoelectric Point: 7.3199

>T26773 WP_013384044.1 NC_017384:c786972-786724 [Ketogulonicigenium vulgare WSH-001]
MMMIVWDEPKRESNLEKHGLDFADLSEEFFLSSVVVPAKNGRHMAIGKLADGTIAVVFATLGTQGLSVISMRHASKKERA
IL

Nucleotide

• Length: 249 bp

>T26773 NC_017384:c786972-786724 [Ketogulonicigenium vulgare WSH-001]
ATGATGATGATTGTCTGGGATGAACCGAAACGCGAAAGCAACCTCGAGAAACACGGGTTAGATTTCGCAGATTTGTCCGA
GGAATTCTTTCTGTCCTCGGTCGTTGTCCCGGCAAAGAACGGGCGTCACATGGCGATAGGGAAGCTTGCCGACGGAACGA
TTGCAGTGGTCTTTGCCACACTTGGAACGCAAGGCCTGTCGGTGATTTCGATGCGCCACGCCAGCAAAAAGGAAAGGGCG
ATCCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10567.07 Da
• Isoelectric Point: 10.4185

>AT26773 WP_013384043.1 NC_017384:c786727-786437 [Ketogulonicigenium vulgare WSH-001]
MKANKLTEKEIQRRIASDPDAPEATDDQLAKAKPFAEAMPQLAENLRRNLGGRPKSDNPKVAVSLRLDQDVIEKFKAAGP
GWQSRMNKALREAAGV

Nucleotide

• Length: 291 bp

>AT26773 NC_017384:c786727-786437 [Ketogulonicigenium vulgare WSH-001]
ATGAAAGCCAACAAGCTAACCGAGAAAGAAATCCAACGCCGGATCGCAAGCGACCCGGACGCACCCGAGGCAACAGATGA
CCAGCTGGCGAAGGCGAAGCCCTTCGCCGAGGCCATGCCCCAACTGGCAGAGAACCTGCGCCGCAACCTTGGCGGCCGCC
CGAAATCCGACAACCCCAAAGTCGCGGTTTCGCTGCGCCTCGATCAAGACGTGATTGAGAAGTTCAAGGCCGCTGGCCCC
GGCTGGCAGTCGCGCATGAACAAAGCCCTGCGCGAAGCTGCGGGGGTATGA

Structures

Antitoxin

• AlphaFold DB: F9Y4N2