Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 8455..8980 Replicon plasmid p_STEC1575_1
Accession NZ_CP116045
Organism Escherichia coli strain STEC1575

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag PHA55_RS24195 Protein ID WP_001159871.1
Coordinates 8455..8760 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PHA55_RS24200 Protein ID WP_000813634.1
Coordinates 8762..8980 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA55_RS24165 (3722) 3722..4992 + 1271 Protein_7 translesion error-prone DNA polymerase V subunit UmuC -
PHA55_RS24170 (4997) 4997..5389 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
PHA55_RS24175 (5394) 5394..6365 - 972 WP_001103694.1 plasmid segregation protein ParM -
PHA55_RS24180 (6594) 6594..7238 + 645 WP_000633911.1 AAA family ATPase -
PHA55_RS24185 (7232) 7232..7507 + 276 WP_000239529.1 hypothetical protein -
PHA55_RS24190 (7645) 7645..8454 - 810 WP_161619134.1 site-specific integrase -
PHA55_RS24195 (8455) 8455..8760 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
PHA55_RS24200 (8762) 8762..8980 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PHA55_RS24205 (9935) 9935..10138 - 204 Protein_15 integrase core domain-containing protein -
PHA55_RS24210 (11208) 11208..11864 + 657 WP_235150763.1 PIG-L family deacetylase -
PHA55_RS24215 (11875) 11875..13071 + 1197 WP_161619135.1 glycosyltransferase family 2 protein -
PHA55_RS24220 (13049) 13049..13630 + 582 WP_096934554.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD 1..88676 88676


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T267722 WP_001159871.1 NZ_CP116045:c8760-8455 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT267722 WP_000813634.1 NZ_CP116045:c8980-8762 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References