Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 519022..519519 Replicon chromosome
Accession NZ_CP116044
Organism Escherichia coli strain STEC1575

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PHA55_RS02550 Protein ID WP_001173343.1
Coordinates 519346..519519 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PHA55_RS02545 Protein ID WP_000398619.1
Coordinates 519022..519318 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA55_RS02525 (515753) 515753..516472 - 720 WP_000611288.1 two-component system response regulator DcuR -
PHA55_RS02530 (516469) 516469..518100 - 1632 WP_001216460.1 sensor histidine kinase -
PHA55_RS02535 (518281) 518281..518511 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
PHA55_RS02540 (518523) 518523..518795 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
PHA55_RS02545 (519022) 519022..519318 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PHA55_RS02550 (519346) 519346..519519 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PHA55_RS02555 (519638) 519638..521155 - 1518 WP_001295074.1 lysine--tRNA ligase -
PHA55_RS02560 (521392) 521392..522849 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T267697 WP_001173343.1 NZ_CP116044:519346-519519 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT267697 WP_000398619.1 NZ_CP116044:519022-519318 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References