267672

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57714..58239 Replicon plasmid unnamed2
Accession NZ_CP116037
Organism Escherichia coli strain FAH

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag PGH49_RS25755 Protein ID WP_001159868.1
Coordinates 57934..58239 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PGH49_RS25750 Protein ID WP_000813634.1
Coordinates 57714..57932 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PGH49_RS25720 (PGH49_25720) 53107..53523 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
PGH49_RS25725 (PGH49_25725) 53520..53750 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
PGH49_RS25730 (PGH49_25730) 54015..54515 + 501 WP_001773886.1 HEPN family nuclease -
PGH49_RS25735 (PGH49_25735) 54528..55301 + 774 WP_000905949.1 hypothetical protein -
PGH49_RS25740 (PGH49_25740) 55468..56601 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
PGH49_RS25745 (PGH49_25745) 56635..57147 - 513 WP_000151784.1 hypothetical protein -
PGH49_RS25750 (PGH49_25750) 57714..57932 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PGH49_RS25755 (PGH49_25755) 57934..58239 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
PGH49_RS25760 (PGH49_25760) 58240..59046 + 807 WP_000016982.1 site-specific integrase -
PGH49_RS25765 (PGH49_25765) 59820..60575 + 756 WP_000852146.1 replication initiation protein RepE -
PGH49_RS25770 (PGH49_25770) 61163..62329 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..82743 82743


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T267672 WP_001159868.1 NZ_CP116037:57934-58239 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT267672 WP_000813634.1 NZ_CP116037:57714-57932 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References