267640

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 19908..21045 Replicon plasmid pFYY063-optrA-70K
Accession NZ_CP116030
Organism Enterococcus casseliflavus strain CQFYY22-063

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag PEZ80_RS16565 Protein ID WP_001284311.1
Coordinates 20182..21045 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag PEZ80_RS16560 Protein ID WP_000301765.1
Coordinates 19908..20180 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PEZ80_RS16545 (PEZ80_16540) 16753..18585 + 1833 WP_256969062.1 type IA DNA topoisomerase -
PEZ80_RS16550 (PEZ80_16545) 18688..19584 + 897 WP_001835294.1 ParA family protein -
PEZ80_RS16555 (PEZ80_16550) 19676..19891 + 216 WP_001835296.1 peptide-binding protein -
PEZ80_RS16560 (PEZ80_16555) 19908..20180 + 273 WP_000301765.1 antitoxin Antitoxin
PEZ80_RS16565 (PEZ80_16560) 20182..21045 + 864 WP_001284311.1 zeta toxin family protein Toxin
PEZ80_RS16570 (PEZ80_16565) 21192..21509 + 318 WP_002300567.1 hypothetical protein -
PEZ80_RS16575 (PEZ80_16570) 22043..22540 + 498 WP_010713926.1 molecular chaperone DnaJ -
PEZ80_RS16580 (PEZ80_16575) 22575..22697 + 123 WP_159111623.1 DpnD/PcfM family protein -
PEZ80_RS16585 (PEZ80_16580) 22751..23431 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
PEZ80_RS16590 (PEZ80_16585) 23487..23870 - 384 Protein_24 N-6 DNA methylase -
PEZ80_RS16595 (PEZ80_16590) 24083..25399 - 1317 WP_271292762.1 ISL3 family transposase -
PEZ80_RS16600 (PEZ80_16595) 25625..25765 - 141 Protein_26 JAB domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fexA / optrA / erm(A) - 1..70094 70094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T267640 WP_001284311.1 NZ_CP116030:20182-21045 [Enterococcus casseliflavus]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT267640 WP_000301765.1 NZ_CP116030:19908-20180 [Enterococcus casseliflavus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References