267632

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 500143..500780 Replicon chromosome
Accession NZ_CP116014
Organism Bacillus velezensis strain SRCM124633

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PF996_RS02510 Protein ID WP_003156187.1
Coordinates 500430..500780 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PF996_RS02505 Protein ID WP_003156188.1
Coordinates 500143..500424 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF996_RS02485 (496508) 496508..497107 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PF996_RS02490 (497200) 497200..497565 + 366 WP_007609580.1 holo-ACP synthase -
PF996_RS02495 (497730) 497730..498737 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PF996_RS02500 (498854) 498854..500023 + 1170 WP_257989017.1 alanine racemase -
PF996_RS02505 (500143) 500143..500424 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PF996_RS02510 (500430) 500430..500780 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PF996_RS02515 (500898) 500898..501719 + 822 WP_257989018.1 STAS domain-containing protein -
PF996_RS02520 (501724) 501724..502089 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PF996_RS02525 (502092) 502092..502493 + 402 WP_003156178.1 anti-sigma regulatory factor -
PF996_RS02530 (502505) 502505..503512 + 1008 WP_257989019.1 PP2C family protein-serine/threonine phosphatase -
PF996_RS02535 (503576) 503576..503905 + 330 WP_016937169.1 anti-sigma factor antagonist -
PF996_RS02540 (503902) 503902..504384 + 483 WP_003156173.1 anti-sigma B factor RsbW -
PF996_RS02545 (504350) 504350..505138 + 789 WP_257989020.1 RNA polymerase sigma factor SigB -
PF996_RS02550 (505138) 505138..505740 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267632 WP_003156187.1 NZ_CP116014:500430-500780 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT267632 WP_003156188.1 NZ_CP116014:500143-500424 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References