Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3635204..3635840 Replicon chromosome
Accession NZ_CP116013
Organism Bacillus velezensis strain SRCM124349

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PF986_RS17980 Protein ID WP_003156187.1
Coordinates 3635490..3635840 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PF986_RS17975 Protein ID WP_003225183.1
Coordinates 3635204..3635485 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF986_RS17955 (3631569) 3631569..3632168 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PF986_RS17960 (3632261) 3632261..3632626 + 366 WP_260654792.1 holo-ACP synthase -
PF986_RS17965 (3632791) 3632791..3633798 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PF986_RS17970 (3633915) 3633915..3635084 + 1170 WP_065180972.1 alanine racemase -
PF986_RS17975 (3635204) 3635204..3635485 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PF986_RS17980 (3635490) 3635490..3635840 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PF986_RS17985 (3635960) 3635960..3636781 + 822 WP_003156182.1 STAS domain-containing protein -
PF986_RS17990 (3636786) 3636786..3637151 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PF986_RS17995 (3637154) 3637154..3637555 + 402 WP_003156178.1 anti-sigma regulatory factor -
PF986_RS18000 (3637567) 3637567..3638574 + 1008 WP_257989019.1 PP2C family protein-serine/threonine phosphatase -
PF986_RS18005 (3638638) 3638638..3638967 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PF986_RS18010 (3638964) 3638964..3639446 + 483 WP_003156173.1 anti-sigma B factor RsbW -
PF986_RS18015 (3639412) 3639412..3640200 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PF986_RS18020 (3640200) 3640200..3640802 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267631 WP_003156187.1 NZ_CP116013:3635490-3635840 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT267631 WP_003225183.1 NZ_CP116013:3635204-3635485 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References