Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 428411..429328 Replicon chromosome
Accession NZ_CP116013
Organism Bacillus velezensis strain SRCM124349

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag PF986_RS01965 Protein ID WP_007407256.1
Coordinates 428582..429328 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag PF986_RS01960 Protein ID WP_003154807.1
Coordinates 428411..428581 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF986_RS01910 (423617) 423617..424129 + 513 WP_017417605.1 sigma-70 family RNA polymerase sigma factor -
PF986_RS01915 (424242) 424242..424793 + 552 Protein_381 terminase -
PF986_RS01920 (424904) 424904..425266 + 363 WP_014721043.1 hypothetical protein -
PF986_RS01925 (425279) 425279..425650 + 372 WP_017417608.1 XkdW family protein -
PF986_RS01930 (425655) 425655..425852 + 198 WP_007610833.1 XkdX family protein -
PF986_RS01935 (425909) 425909..426670 + 762 WP_025649639.1 hypothetical protein -
PF986_RS01940 (426722) 426722..426985 + 264 WP_021493821.1 hemolysin XhlA family protein -
PF986_RS01945 (426999) 426999..427262 + 264 WP_003154813.1 phage holin -
PF986_RS01950 (427276) 427276..428154 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
PF986_RS01955 (428189) 428189..428314 - 126 WP_003154809.1 hypothetical protein -
PF986_RS01960 (428411) 428411..428581 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
PF986_RS01965 (428582) 428582..429328 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
PF986_RS01970 (429432) 429432..430430 - 999 WP_017417610.1 inorganic phosphate transporter -
PF986_RS01975 (430443) 430443..431060 - 618 WP_003154804.1 DUF47 domain-containing protein -
PF986_RS01980 (431347) 431347..432663 - 1317 WP_007610842.1 amino acid permease -
PF986_RS01985 (432985) 432985..433935 + 951 WP_007610844.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T267630 WP_007407256.1 NZ_CP116013:c429328-428582 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT267630 WP_003154807.1 NZ_CP116013:c428581-428411 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References