267629

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3788769..3789405 Replicon chromosome
Accession NZ_CP116012
Organism Bacillus subtilis strain SRCM124333

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PF977_RS20085 Protein ID WP_003156187.1
Coordinates 3788769..3789119 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag PF977_RS20090 Protein ID WP_003225183.1
Coordinates 3789124..3789405 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF977_RS20045 (3783813) 3783813..3784412 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
PF977_RS20050 (3784412) 3784412..3785200 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
PF977_RS20055 (3785166) 3785166..3785648 - 483 WP_003234299.1 anti-sigma B factor RsbW -
PF977_RS20060 (3785645) 3785645..3785974 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
PF977_RS20065 (3786036) 3786036..3787043 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
PF977_RS20070 (3787055) 3787055..3787456 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
PF977_RS20075 (3787460) 3787460..3787825 - 366 WP_015715277.1 RsbT antagonist protein RsbS -
PF977_RS20080 (3787830) 3787830..3788654 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
PF977_RS20085 (3788769) 3788769..3789119 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PF977_RS20090 (3789124) 3789124..3789405 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PF977_RS20095 (3789521) 3789521..3790690 - 1170 WP_003234284.1 alanine racemase -
PF977_RS20100 (3790805) 3790805..3791821 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
PF977_RS20105 (3791987) 3791987..3792352 - 366 WP_003234281.1 holo-ACP synthase -
PF977_RS20110 (3792447) 3792447..3793046 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267629 WP_003156187.1 NZ_CP116012:c3789119-3788769 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT267629 WP_003225183.1 NZ_CP116012:c3789405-3789124 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References