Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 434334..434971 Replicon chromosome
Accession NZ_CP116011
Organism Bacillus amyloliquefaciens strain SRCM124317

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PF976_RS02235 Protein ID WP_003156187.1
Coordinates 434621..434971 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PF976_RS02230 Protein ID WP_003156188.1
Coordinates 434334..434615 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF976_RS02210 (430700) 430700..431299 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
PF976_RS02215 (431392) 431392..431757 + 366 WP_013351093.1 holo-ACP synthase -
PF976_RS02220 (431922) 431922..432929 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
PF976_RS02225 (433046) 433046..434215 + 1170 WP_013351095.1 alanine racemase -
PF976_RS02230 (434334) 434334..434615 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PF976_RS02235 (434621) 434621..434971 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PF976_RS02240 (435092) 435092..435913 + 822 WP_013351096.1 STAS domain-containing protein -
PF976_RS02245 (435918) 435918..436283 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
PF976_RS02250 (436286) 436286..436687 + 402 WP_013351097.1 anti-sigma regulatory factor -
PF976_RS02255 (436699) 436699..437706 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
PF976_RS02260 (437770) 437770..438099 + 330 WP_014469786.1 anti-sigma factor antagonist -
PF976_RS02265 (438096) 438096..438578 + 483 WP_013351100.1 anti-sigma B factor RsbW -
PF976_RS02270 (438544) 438544..439332 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
PF976_RS02275 (439332) 439332..439934 + 603 WP_014469787.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267617 WP_003156187.1 NZ_CP116011:434621-434971 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT267617 WP_003156188.1 NZ_CP116011:434334-434615 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References