267616

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3548302..3548939 Replicon chromosome
Accession NZ_CP116010
Organism Bacillus velezensis strain SRCM123815

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PF975_RS17420 Protein ID WP_003156187.1
Coordinates 3548302..3548652 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PF975_RS17425 Protein ID WP_003156188.1
Coordinates 3548658..3548939 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF975_RS17380 (PF975_17380) 3543342..3543944 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
PF975_RS17385 (PF975_17385) 3543944..3544732 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PF975_RS17390 (PF975_17390) 3544698..3545180 - 483 WP_007609591.1 anti-sigma B factor RsbW -
PF975_RS17395 (PF975_17395) 3545177..3545506 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PF975_RS17400 (PF975_17400) 3545570..3546577 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
PF975_RS17405 (PF975_17405) 3546589..3546990 - 402 WP_003156178.1 anti-sigma regulatory factor -
PF975_RS17410 (PF975_17410) 3546993..3547358 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
PF975_RS17415 (PF975_17415) 3547363..3548184 - 822 WP_003156182.1 STAS domain-containing protein -
PF975_RS17420 (PF975_17420) 3548302..3548652 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PF975_RS17425 (PF975_17425) 3548658..3548939 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PF975_RS17430 (PF975_17430) 3549059..3550228 - 1170 WP_053285486.1 alanine racemase -
PF975_RS17435 (PF975_17435) 3550345..3551352 - 1008 WP_021495080.1 outer membrane lipoprotein carrier protein LolA -
PF975_RS17440 (PF975_17440) 3551517..3551882 - 366 WP_014304402.1 holo-ACP synthase -
PF975_RS17445 (PF975_17445) 3551975..3552574 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267616 WP_003156187.1 NZ_CP116010:c3548652-3548302 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT267616 WP_003156188.1 NZ_CP116010:c3548939-3548658 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References