Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 26489..27108 Replicon plasmid pESC1
Accession NZ_CP115993
Organism Enterococcus faecalis strain ESC1

Toxin (Protein)


Gene name mazF Uniprot ID R3H4V2
Locus tag PE069_RS14145 Protein ID WP_000241511.1
Coordinates 26489..26851 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3H5D1
Locus tag PE069_RS14150 Protein ID WP_000245205.1
Coordinates 26845..27108 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PE069_RS14130 (PE069_14130) 21899..23830 - 1932 WP_010816308.1 sucrose-specific PTS transporter subunit IIBC -
PE069_RS14135 (PE069_14135) 24021..25460 + 1440 WP_002367771.1 sucrose-6-phosphate hydrolase -
PE069_RS14140 (PE069_14140) 25462..26424 + 963 WP_002367770.1 LacI family DNA-binding transcriptional regulator -
PE069_RS14145 (PE069_14145) 26489..26851 - 363 WP_000241511.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PE069_RS14150 (PE069_14150) 26845..27108 - 264 WP_000245205.1 PbsX family transcriptional regulator Antitoxin
PE069_RS14155 (PE069_14155) 27435..28799 - 1365 WP_159156973.1 SEC-C metal-binding domain-containing protein -
PE069_RS14160 (PE069_14160) 29225..29311 - 87 WP_271232472.1 putative holin-like toxin -
PE069_RS14165 (PE069_14165) 29436..29597 - 162 WP_181039656.1 hypothetical protein -
PE069_RS14170 (PE069_14170) 29610..30200 - 591 WP_159156972.1 thermonuclease family protein -
PE069_RS14175 (PE069_14175) 30298..30747 - 450 WP_104801932.1 single-stranded DNA-binding protein -
PE069_RS14180 (PE069_14180) 30872..31069 + 198 WP_014862324.1 hypothetical protein -
PE069_RS14185 (PE069_14185) 31145..31408 + 264 WP_010817818.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..73156 73156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13585.78 Da        Isoelectric Point: 9.6107

>T267586 WP_000241511.1 NZ_CP115993:c26851-26489 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Download         Length: 363 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10059.46 Da        Isoelectric Point: 5.1585

>AT267586 WP_000245205.1 NZ_CP115993:c27108-26845 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3H4V2

Antitoxin

Source ID Structure
AlphaFold DB R3H5D1

References