267585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 8168..8739 Replicon plasmid pESC1
Accession NZ_CP115993
Organism Enterococcus faecalis strain ESC1

Toxin (Protein)


Gene name mazF Uniprot ID A0A2P6BPI5
Locus tag PE069_RS14065 Protein ID WP_002394791.1
Coordinates 8398..8739 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag PE069_RS14060 Protein ID WP_002362431.1
Coordinates 8168..8398 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PE069_RS14030 (PE069_14030) 3358..3966 - 609 WP_224798875.1 polysaccharide deacetylase family protein -
PE069_RS14035 (PE069_14035) 4301..5821 - 1521 WP_271232469.1 hypothetical protein -
PE069_RS14040 (PE069_14040) 6575..6760 - 186 WP_169061676.1 DNA helicase UvrB -
PE069_RS14045 (PE069_14045) 6822..7046 - 225 WP_010711817.1 hypothetical protein -
PE069_RS14050 (PE069_14050) 7040..7327 - 288 Protein_10 hypothetical protein -
PE069_RS14055 (PE069_14055) 7344..7964 - 621 WP_163326451.1 recombinase family protein -
PE069_RS14060 (PE069_14060) 8168..8398 + 231 WP_002362431.1 hypothetical protein Antitoxin
PE069_RS14065 (PE069_14065) 8398..8739 + 342 WP_002394791.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PE069_RS14070 (PE069_14070) 8851..9789 + 939 WP_002394789.1 hypothetical protein -
PE069_RS14075 (PE069_14075) 10057..10659 + 603 WP_271232470.1 Fic family protein -
PE069_RS14080 (PE069_14080) 10893..11573 - 681 WP_002367779.1 IS6 family transposase -
PE069_RS14085 (PE069_14085) 11828..12766 + 939 WP_002335374.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..73156 73156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13248.49 Da        Isoelectric Point: 8.8595

>T267585 WP_002394791.1 NZ_CP115993:8398-8739 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT267585 WP_002362431.1 NZ_CP115993:8168-8398 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P6BPI5

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References