Detailed information of TA system TA267533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1841249..1842705
• Replicon: plasmid pmega
• Accession: NZ_CP115947
• Organism: Ralstonia solanacearum MolK2

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: PG909_RS22360
• Protein ID: WP_003274021.1
• Coordinates: 1841860..1842705 (+)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: PG909_RS22355
• Protein ID: WP_003274020.1
• Coordinates: 1841249..1841863 (+)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PG909_RS22335	1837049..1838264	+	1216	Protein_1692	chitinase	-
PG909_RS22340	1838334..1840070	-	1737	WP_003274018.1	gamma-glutamyltransferase	-
PG909_RS22350	1840801..1841128	-	328	Protein_1694	DUF932 domain-containing protein	-
PG909_RS22355	1841249..1841863	+	615	WP_003274020.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
PG909_RS22360	1841860..1842705	+	846	WP_003274021.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
PG909_RS22365	1842869..1843783	-	915	WP_003274022.1	transcriptional regulator GcvA	-
PG909_RS22370	1843936..1844712	+	777	WP_003274788.1	SDR family NAD(P)-dependent oxidoreductase	-
PG909_RS22375	1844738..1845019	+	282	WP_003274790.1	hypothetical protein	-
PG909_RS22380	1845054..1845398	+	345	WP_003274026.1	carboxymuconolactone decarboxylase family protein	-
PG909_RS22385	1845464..1846144	+	681	WP_042550389.1	cupin domain-containing protein	-
PG909_RS22390	1846138..1847256	+	1119	WP_003274028.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / icl	1..2326525	2326525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-259)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-55)

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 31302.80 Da
• Isoelectric Point: 7.5126

>T267533 WP_003274021.1 NZ_CP115947:1841860-1842705 [Ralstonia solanacearum MolK2]
MSRNLAASIRTRLKQHADASKQDFNLTLTQYGLERLLYRLSISQHAANYLLKGALLFALWYDQPHRPTRDADLLGYGPDD
IDTAVAAFREISRIEVEDGIAFEPASIRGLEIRKETGYGGVRIDLQAKLDGARIALQVDIGFGDAVTPAPETVDYPTLLD
NLPAPRLRAYPKHTVVAEKLHAVCLLGMANTRMKDYFDLWVLLTEDTLDPGELRRAAQATFTRRGLAIPEATPAGLSDTF
ARDATKQQQWAAFLKKNRLPAMDLAQVVALLRNAFQHLQAH

Nucleotide

• Length: 846 bp

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 22686.52 Da
• Isoelectric Point: 10.3527

>AT267533 WP_003274020.1 NZ_CP115947:1841249-1841863 [Ralstonia solanacearum MolK2]
MTLPHQTLEQAVLALAERQPLLRARDLAKRGLPTMVLSRLVAAGKLERIARGIYSLQGRALGEHRSLAESALRVPRGVVC
LLSALRVHGIGTQAPFEVWMAIPPHTPTPSLDQPALRVVRMSGAAMTEGIEHVEIDGVPVPLFNLDKTVADCFKYRNKIG
LDVALEALRDGWSQRRLTMDRLWHYATINRVTNVMRPYLESVTT

Nucleotide

• Length: 615 bp