Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5249728..5250353 Replicon chromosome
Accession NZ_CP115930
Organism Klebsiella pneumoniae subsp. pneumoniae strain 43UC01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DLD89_RS25980 Protein ID WP_002882817.1
Coordinates 5249728..5250111 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DLD89_RS25985 Protein ID WP_004150355.1
Coordinates 5250111..5250353 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLD89_RS25965 (DLD89_25960) 5247094..5247996 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DLD89_RS25970 (DLD89_25965) 5247993..5248628 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DLD89_RS25975 (DLD89_25970) 5248625..5249554 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DLD89_RS25980 (DLD89_25975) 5249728..5250111 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DLD89_RS25985 (DLD89_25980) 5250111..5250353 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DLD89_RS25990 (DLD89_25985) 5250558..5251475 + 918 WP_002882812.1 alpha/beta hydrolase -
DLD89_RS25995 (DLD89_25990) 5251489..5252430 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DLD89_RS26000 (DLD89_25995) 5252475..5252912 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DLD89_RS26005 (DLD89_26000) 5252909..5253769 - 861 WP_002882807.1 virulence factor BrkB family protein -
DLD89_RS26010 (DLD89_26005) 5253763..5254362 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T267510 WP_002882817.1 NZ_CP115930:c5250111-5249728 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT267510 WP_004150355.1 NZ_CP115930:c5250353-5250111 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References