Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 296820..297610 Replicon plasmid pAtK224a
Accession NZ_CP115926
Organism Agrobacterium fabacearum strain K224

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1S7NFF1
Locus tag G6L41_RS24250 Protein ID WP_019565755.1
Coordinates 297113..297610 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1S7NER6
Locus tag G6L41_RS24245 Protein ID WP_019565754.1
Coordinates 296820..297116 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6L41_RS24225 (G6L41_024225) 292188..293912 + 1725 WP_173994789.1 ABC transporter ATP-binding protein -
G6L41_RS24230 (G6L41_024230) 293957..295144 + 1188 WP_013637437.1 galactarate dehydratase -
G6L41_RS24235 (G6L41_024235) 295131..295997 + 867 WP_112497507.1 aldose 1-epimerase -
G6L41_RS24240 (G6L41_024240) 296207..296637 + 431 Protein_287 DDE-type integrase/transposase/recombinase -
G6L41_RS24245 (G6L41_024245) 296820..297116 + 297 WP_019565754.1 DUF1778 domain-containing protein Antitoxin
G6L41_RS24250 (G6L41_024250) 297113..297610 + 498 WP_019565755.1 GNAT family N-acetyltransferase Toxin
G6L41_RS24255 (G6L41_024255) 297889..298554 - 666 WP_236762315.1 GNAT family N-acetyltransferase -
G6L41_RS24260 (G6L41_024260) 298520..299680 - 1161 WP_173994787.1 Gfo/Idh/MocA family oxidoreductase -
G6L41_RS24265 (G6L41_024265) 299712..300962 - 1251 WP_173994786.1 ROK family transcriptional regulator -
G6L41_RS24270 (G6L41_024270) 301175..302134 + 960 WP_111801318.1 extracellular solute-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..485342 485342


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-142)

Antitoxin

Vibrio phage ICP1, Orf50

(16-90)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17725.27 Da        Isoelectric Point: 6.8629

>T267496 WP_019565755.1 NZ_CP115926:297113-297610 [Agrobacterium fabacearum]
VTLSAPVPLADHHELAEFNSGVPELDDWLRRRARANQAGGASRTFVVCDESRVIAYYALASGSVKPPEVPGRFRRNMPDP
IPVAVLGRLAIDQSCQGRGIGRALVRDAGLRLLNAAEVLGIRGVLVHAISDDARAFYEAVGFLSSPSDPMMLMVGLHDLN
NALNT

Download         Length: 498 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11022.59 Da        Isoelectric Point: 7.0159

>AT267496 WP_019565754.1 NZ_CP115926:296820-297116 [Agrobacterium fabacearum]
MPTSHTGKSPDASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIVTVSPEIYAEYLARLDAPAQ
PNERLKRTMSTKAPWDEA

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S7NFF1

Antitoxin

Source ID Structure
AlphaFold DB A0A1S7NER6

References