Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2261645..2262384 Replicon chromosome
Accession NZ_CP115874
Organism Enterobacter hormaechei subsp. steigerwaltii strain WYTS.MG41

Toxin (Protein)


Gene name tacT Uniprot ID A0A822W909
Locus tag PBS85_RS10655 Protein ID WP_017382345.1
Coordinates 2261645..2262130 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag PBS85_RS10660 Protein ID WP_003857131.1
Coordinates 2262118..2262384 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PBS85_RS10625 (PBS85_10625) 2256675..2257433 + 759 WP_015570520.1 DeoR/GlpR family DNA-binding transcription regulator -
PBS85_RS10630 (PBS85_10630) 2257538..2258830 + 1293 WP_271139147.1 glycosyl hydrolase family 28 protein -
PBS85_RS10635 (PBS85_10635) 2258899..2259444 + 546 WP_032671141.1 NUDIX hydrolase -
PBS85_RS10640 (PBS85_10640) 2259628..2260263 - 636 WP_015570517.1 DUF421 domain-containing protein -
PBS85_RS10645 (PBS85_10645) 2260273..2260719 - 447 WP_014069654.1 DUF3290 domain-containing protein -
PBS85_RS10650 (PBS85_10650) 2261148..2261464 - 317 Protein_2074 ISNCY family transposase -
PBS85_RS10655 (PBS85_10655) 2261645..2262130 - 486 WP_017382345.1 GNAT family N-acetyltransferase Toxin
PBS85_RS10660 (PBS85_10660) 2262118..2262384 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
PBS85_RS10665 (PBS85_10665) 2262448..2263377 - 930 WP_271139148.1 LysR family transcriptional regulator -
PBS85_RS10670 (PBS85_10670) 2263507..2264895 + 1389 WP_032619651.1 MFS transporter -
PBS85_RS10675 (PBS85_10675) 2264917..2265912 - 996 WP_023303746.1 DUF2891 domain-containing protein -
PBS85_RS10680 (PBS85_10680) 2265922..2266908 - 987 WP_017382341.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2246262..2262384 16122


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17510.21 Da        Isoelectric Point: 9.9658

>T267446 WP_017382345.1 NZ_CP115874:c2262130-2261645 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARAFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT267446 WP_003857131.1 NZ_CP115874:c2262384-2262118 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822W909

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References