Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5201992..5202617 Replicon chromosome
Accession NZ_CP115837
Organism Klebsiella pneumoniae strain MRSN22265

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KFB76_RS25460 Protein ID WP_002882817.1
Coordinates 5201992..5202375 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFB76_RS25465 Protein ID WP_004150355.1
Coordinates 5202375..5202617 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFB76_RS25445 (KFB76_025440) 5199358..5200260 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KFB76_RS25450 (KFB76_025445) 5200257..5200892 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFB76_RS25455 (KFB76_025450) 5200889..5201818 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFB76_RS25460 (KFB76_025455) 5201992..5202375 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KFB76_RS25465 (KFB76_025460) 5202375..5202617 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFB76_RS25470 (KFB76_025465) 5202822..5203739 + 918 WP_009484979.1 alpha/beta hydrolase -
KFB76_RS25475 (KFB76_025470) 5203753..5204694 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFB76_RS25480 (KFB76_025475) 5204739..5205176 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFB76_RS25485 (KFB76_025480) 5205173..5206033 - 861 WP_004146232.1 virulence factor BrkB family protein -
KFB76_RS25490 (KFB76_025485) 5206027..5206626 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T267432 WP_002882817.1 NZ_CP115837:c5202375-5201992 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT267432 WP_004150355.1 NZ_CP115837:c5202617-5202375 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References