Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1805747..1806337 Replicon chromosome
Accession NZ_CP115837
Organism Klebsiella pneumoniae strain MRSN22265

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag KFB76_RS08770 Protein ID WP_023341911.1
Coordinates 1806005..1806337 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag KFB76_RS08765 Protein ID WP_000288812.1
Coordinates 1805747..1806004 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFB76_RS08735 (KFB76_008735) 1801013..1801710 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
KFB76_RS08740 (KFB76_008740) 1801721..1802335 - 615 WP_271294308.1 ATP-binding protein -
KFB76_RS08745 (KFB76_008745) 1802347..1803696 - 1350 WP_077254247.1 DNA (cytosine-5-)-methyltransferase -
KFB76_RS08750 (KFB76_008750) 1803704..1804120 - 417 WP_124047552.1 hypothetical protein -
KFB76_RS08755 (KFB76_008755) 1804748..1805209 + 462 WP_040210976.1 hypothetical protein -
KFB76_RS08760 (KFB76_008760) 1805206..1805412 + 207 WP_022615589.1 helix-turn-helix transcriptional regulator -
KFB76_RS08765 (KFB76_008765) 1805747..1806004 + 258 WP_000288812.1 antitoxin Antitoxin
KFB76_RS08770 (KFB76_008770) 1806005..1806337 + 333 WP_023341911.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFB76_RS08780 (KFB76_008780) 1806659..1808095 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KFB76_RS08790 (KFB76_008790) 1808461..1809915 - 1455 WP_004148975.1 AMP nucleosidase -
KFB76_RS08795 (KFB76_008795) 1810045..1810290 - 246 WP_023284006.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1801207..1801710 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11927.87 Da        Isoelectric Point: 10.4722

>T267421 WP_023341911.1 NZ_CP115837:1806005-1806337 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT267421 WP_000288812.1 NZ_CP115837:1805747-1806004 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References