Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1458342..1459078 Replicon chromosome
Accession NZ_CP115837
Organism Klebsiella pneumoniae strain MRSN22265

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag KFB76_RS07220 Protein ID WP_032433360.1
Coordinates 1458596..1459078 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KFB76_RS07215 Protein ID WP_003026799.1
Coordinates 1458342..1458608 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFB76_RS07190 (KFB76_007190) 1453988..1455127 + 1140 WP_040025615.1 mannitol dehydrogenase -
KFB76_RS07195 (KFB76_007195) 1455156..1455818 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
KFB76_RS07200 (KFB76_007200) 1455802..1456806 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
KFB76_RS07205 (KFB76_007205) 1456824..1457456 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
KFB76_RS07210 (KFB76_007210) 1457466..1458029 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
KFB76_RS07215 (KFB76_007215) 1458342..1458608 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KFB76_RS07220 (KFB76_007220) 1458596..1459078 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
KFB76_RS07225 (KFB76_007225) 1459439..1460038 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
KFB76_RS07230 (KFB76_007230) 1460250..1461194 - 945 WP_077254249.1 fimbrial protein -
KFB76_RS07235 (KFB76_007235) 1461206..1461784 - 579 WP_032432061.1 type 1 fimbrial protein -
KFB76_RS07240 (KFB76_007240) 1461788..1462528 - 741 WP_048978466.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1430642..1470019 39377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T267420 WP_032433360.1 NZ_CP115837:1458596-1459078 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT267420 WP_003026799.1 NZ_CP115837:1458342-1458608 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References