Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 88887..89638 Replicon plasmid pL4FAA5_1
Accession NZ_CP115832
Organism Klebsiella pneumoniae strain L4-FAA5

Toxin (Protein)


Gene name ataT Uniprot ID W9BA31
Locus tag PGO70_RS25815 Protein ID WP_032104592.1
Coordinates 88887..89369 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID W9B1S8
Locus tag PGO70_RS25820 Protein ID WP_016529519.1
Coordinates 89360..89638 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PGO70_RS25785 (PGO70_25780) 84470..84922 + 453 WP_020948374.1 transcriptional repressor -
PGO70_RS25790 (PGO70_25785) 85070..86298 + 1229 WP_085956323.1 IS3 family transposase -
PGO70_RS25795 (PGO70_25790) 86683..86919 + 237 WP_016529523.1 hypothetical protein -
PGO70_RS25800 (PGO70_25795) 87014..87175 - 162 WP_223367960.1 bacteriocin immunity protein -
PGO70_RS25805 (PGO70_25800) 87275..88108 - 834 WP_012540133.1 GIY-YIG nuclease family protein -
PGO70_RS25810 (PGO70_25805) 88445..88849 - 405 WP_016529521.1 DUF2251 domain-containing protein -
PGO70_RS25815 (PGO70_25810) 88887..89369 - 483 WP_032104592.1 GNAT family N-acetyltransferase Toxin
PGO70_RS25820 (PGO70_25815) 89360..89638 - 279 WP_016529519.1 DUF1778 domain-containing protein Antitoxin
PGO70_RS25825 (PGO70_25820) 89946..90482 + 537 WP_032445791.1 hypothetical protein -
PGO70_RS25830 (PGO70_25825) 90866..91876 - 1011 WP_004152284.1 zinc-binding alcohol dehydrogenase family protein -
PGO70_RS25835 (PGO70_25830) 92219..92301 + 83 Protein_98 hypothetical protein -
PGO70_RS25840 (PGO70_25835) 92337..93419 + 1083 WP_016528990.1 DNA-binding transcriptional repressor LacI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - rmpA / rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..162142 162142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(6-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17781.73 Da        Isoelectric Point: 9.4946

>T267413 WP_032104592.1 NZ_CP115832:c89369-88887 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVLSEWLKKKALKNHRTGISRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Download         Length: 483 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10708.23 Da        Isoelectric Point: 4.5156

>AT267413 WP_016529519.1 NZ_CP115832:c89638-89360 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDNQFNAFVEELDAPMTPERQAR
IDNLMSRKSPWE

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BA31

Antitoxin

Source ID Structure
AlphaFold DB W9B1S8

References