Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5176977..5177602 Replicon chromosome
Accession NZ_CP115714
Organism Klebsiella pneumoniae strain KPN0421

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PF051_RS25255 Protein ID WP_002882817.1
Coordinates 5176977..5177360 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PF051_RS25260 Protein ID WP_004150355.1
Coordinates 5177360..5177602 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PF051_RS25240 (5174343) 5174343..5175245 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PF051_RS25245 (5175242) 5175242..5175877 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PF051_RS25250 (5175874) 5175874..5176803 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PF051_RS25255 (5176977) 5176977..5177360 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PF051_RS25260 (5177360) 5177360..5177602 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PF051_RS25265 (5177807) 5177807..5178724 + 918 WP_004150353.1 alpha/beta hydrolase -
PF051_RS25270 (5178738) 5178738..5179679 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PF051_RS25275 (5179724) 5179724..5180161 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PF051_RS25280 (5180158) 5180158..5181018 - 861 WP_004146232.1 virulence factor BrkB family protein -
PF051_RS25285 (5181012) 5181012..5181611 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T267354 WP_002882817.1 NZ_CP115714:c5177360-5176977 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT267354 WP_004150355.1 NZ_CP115714:c5177602-5177360 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References