Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5201166..5201791 Replicon chromosome
Accession NZ_CP115707
Organism Klebsiella pneumoniae strain CPE16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag PGD48_RS25250 Protein ID WP_002882817.1
Coordinates 5201166..5201549 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag PGD48_RS25255 Protein ID WP_004150355.1
Coordinates 5201549..5201791 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PGD48_RS25235 (5198532) 5198532..5199434 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
PGD48_RS25240 (5199431) 5199431..5200066 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
PGD48_RS25245 (5200063) 5200063..5200992 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
PGD48_RS25250 (5201166) 5201166..5201549 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
PGD48_RS25255 (5201549) 5201549..5201791 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
PGD48_RS25260 (5201996) 5201996..5202913 + 918 WP_004181612.1 alpha/beta hydrolase -
PGD48_RS25265 (5202927) 5202927..5203868 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
PGD48_RS25270 (5203913) 5203913..5204350 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
PGD48_RS25275 (5204347) 5204347..5205207 - 861 WP_004146232.1 virulence factor BrkB family protein -
PGD48_RS25280 (5205201) 5205201..5205800 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T267315 WP_002882817.1 NZ_CP115707:c5201549-5201166 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT267315 WP_004150355.1 NZ_CP115707:c5201791-5201549 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References