Detailed information of TA system TA267265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 635369..635945
• Replicon: chromosome
• Accession: NZ_CP115689
• Organism: Enterobacter hormaechei strain 2020CK-00204

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: PF325_RS03020
• Protein ID: WP_015572580.1
• Coordinates: 635369..635656 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: PF325_RS03025
• Protein ID: WP_017694570.1
• Coordinates: 635643..635945 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PF325_RS02995 (PF325_02995)	630462..631181	+	720	WP_108394133.1	winged helix-turn-helix domain-containing protein	-
PF325_RS03000 (PF325_03000)	631331..631801	+	471	WP_015572576.1	MarR family transcriptional regulator	-
PF325_RS03005 (PF325_03005)	631798..632865	+	1068	WP_026080654.1	HlyD family secretion protein	-
PF325_RS03010 (PF325_03010)	632855..633931	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
PF325_RS03015 (PF325_03015)	633928..635199	-	1272	WP_252051980.1	DUF445 domain-containing protein	-
PF325_RS03020 (PF325_03020)	635369..635656	+	288	WP_015572580.1	BrnT family toxin	Toxin
PF325_RS03025 (PF325_03025)	635643..635945	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
PF325_RS03030 (PF325_03030)	635974..636612	-	639	WP_017694569.1	LysE family translocator	-
PF325_RS03035 (PF325_03035)	636651..637403	-	753	WP_015572582.1	AraC family transcriptional regulator	-
PF325_RS03040 (PF325_03040)	637557..638927	+	1371	WP_017694568.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
PF325_RS03045 (PF325_03045)	639105..639650	-	546	WP_006810254.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T267265 WP_015572580.1 NZ_CP115689:635369-635656 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT267265 WP_017694570.1 NZ_CP115689:635643-635945 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp