267232

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 4502728..4503767 Replicon chromosome
Accession NZ_CP115663
Organism Pseudomonas putida strain PD584_L3

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag PED35_RS20505 Protein ID WP_010954922.1
Coordinates 4503192..4503767 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag PED35_RS20500 Protein ID WP_049588231.1
Coordinates 4502728..4503195 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PED35_RS20485 (PED35_20485) 4498880..4499953 + 1074 WP_010954918.1 microcin C ABC transporter permease YejB -
PED35_RS20490 (PED35_20490) 4499955..4500974 + 1020 WP_010954919.1 ABC transporter permease -
PED35_RS20495 (PED35_20495) 4500976..4502580 + 1605 WP_010954920.1 ABC transporter ATP-binding protein -
PED35_RS20500 (PED35_20500) 4502728..4503195 + 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
PED35_RS20505 (PED35_20505) 4503192..4503767 + 576 WP_010954922.1 PIN domain-containing protein Toxin
PED35_RS20510 (PED35_20510) 4503981..4506911 + 2931 WP_049588229.1 aminotransferase -
PED35_RS20515 (PED35_20515) 4506915..4508138 - 1224 WP_010954925.1 NAD(P)/FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T267232 WP_010954922.1 NZ_CP115663:4503192-4503767 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT267232 WP_049588231.1 NZ_CP115663:4502728-4503195 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References