Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 2699967..2700850 Replicon chromosome
Accession NZ_CP115663
Organism Pseudomonas putida strain PD584_L3

Toxin (Protein)


Gene name - Uniprot ID Q88K57
Locus tag PED35_RS12240 Protein ID WP_003250527.1
Coordinates 2700413..2700850 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID I7C3Q7
Locus tag PED35_RS12235 Protein ID WP_003250525.1
Coordinates 2699967..2700416 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PED35_RS12210 (PED35_12210) 2695391..2696590 + 1200 WP_049587621.1 sugar transporter -
PED35_RS12215 (PED35_12215) 2696630..2697214 - 585 WP_010953382.1 LysE family transporter -
PED35_RS12220 (PED35_12220) 2697265..2698095 - 831 WP_003250518.1 AraC family transcriptional regulator -
PED35_RS12225 (PED35_12225) 2698144..2699067 - 924 WP_010953383.1 LysR family transcriptional regulator -
PED35_RS12230 (PED35_12230) 2699171..2699824 + 654 WP_010953384.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
PED35_RS12235 (PED35_12235) 2699967..2700416 + 450 WP_003250525.1 DUF2384 domain-containing protein Antitoxin
PED35_RS12240 (PED35_12240) 2700413..2700850 + 438 WP_003250527.1 RES family NAD+ phosphorylase Toxin
PED35_RS12245 (PED35_12245) 2700858..2702012 - 1155 WP_049587669.1 aminotransferase class V-fold PLP-dependent enzyme -
PED35_RS12250 (PED35_12250) 2702206..2703132 - 927 WP_010953387.1 LysR family transcriptional regulator -
PED35_RS12255 (PED35_12255) 2703273..2704502 + 1230 WP_049587623.1 acyl-CoA dehydrogenase -
PED35_RS12260 (PED35_12260) 2704666..2704914 + 249 Protein_2374 PAAR domain-containing protein -
PED35_RS12265 (PED35_12265) 2704917..2705306 + 390 WP_049587628.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15990.29 Da        Isoelectric Point: 5.6007

>T267230 WP_003250527.1 NZ_CP115663:2700413-2700850 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16956.46 Da        Isoelectric Point: 5.7258

>AT267230 WP_003250525.1 NZ_CP115663:2699967-2700416 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GW6

Antitoxin

Source ID Structure
AlphaFold DB I7C3Q7

References