Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-higA/HigB-HigA
Location 334684..335675 Replicon chromosome
Accession NZ_CP115663
Organism Pseudomonas putida strain PD584_L3

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag PED35_RS01455 Protein ID WP_020190010.1
Coordinates 334684..335160 (+) Length 159 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q88R60
Locus tag PED35_RS01460 Protein ID WP_004575922.1
Coordinates 335202..335675 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PED35_RS01435 (PED35_01435) 329827..331194 - 1368 WP_010951639.1 ATP-binding protein -
PED35_RS01440 (PED35_01440) 331196..331915 - 720 WP_010951640.1 response regulator -
PED35_RS01445 (PED35_01445) 332056..334353 + 2298 WP_010951641.1 TonB-dependent siderophore receptor -
PED35_RS01450 (PED35_01450) 334419..334625 - 207 WP_014860378.1 DUF3079 domain-containing protein -
PED35_RS01455 (PED35_01455) 334684..335160 + 477 WP_020190010.1 type II toxin-antitoxin system HigB family toxin Toxin
PED35_RS01460 (PED35_01460) 335202..335675 + 474 WP_004575922.1 transcriptional regulator Antitoxin
PED35_RS01465 (PED35_01465) 335680..335867 - 188 Protein_282 integrase -
PED35_RS01470 (PED35_01470) 336095..336310 + 216 WP_010951644.1 type II toxin-antitoxin system RelE/ParE family toxin -
PED35_RS01475 (PED35_01475) 336307..336630 - 324 WP_010951645.1 helix-turn-helix transcriptional regulator -
PED35_RS01485 (PED35_01485) 337092..337340 - 249 WP_010951646.1 hypothetical protein -
PED35_RS01490 (PED35_01490) 337861..338136 + 276 WP_049588069.1 DUF3077 domain-containing protein -
PED35_RS01495 (PED35_01495) 338491..339696 - 1206 WP_010951648.1 methyltransferase -
PED35_RS01500 (PED35_01500) 339825..340514 - 690 WP_010951649.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(114-152)

Antitoxin

Antitoxin HigA

(43-154)


Sequences

Toxin        


Download         Length: 159 a.a.        Molecular weight: 18386.38 Da        Isoelectric Point: 10.3522

>T267227 WP_020190010.1 NZ_CP115663:334684-335160 [Pseudomonas putida]
MTGINPLAIARSPYIAHPDIWLTDIWIGDIFHPMLVRRDNTMRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFES
FEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Download         Length: 477 bp

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17229.67 Da        Isoelectric Point: 4.4982

>AT267227 WP_004575922.1 NZ_CP115663:335202-335675 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Download         Length: 474 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CBJ4

References