Detailed information of TA system TA026720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2104148..2104662
• Replicon: chromosome
• Accession: NC_017349
• Organism: Staphylococcus aureus subsp. aureus LGA251

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SARLGA251_RS10420
• Protein ID: WP_000581792.1
• Coordinates: 2104148..2104357 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: SARLGA251_RS10425
• Protein ID: WP_000138934.1
• Coordinates: 2104369..2104662 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SARLGA251_RS10400	2099656..2101014	-	1359	WP_000611474.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SARLGA251_RS10405	2101029..2102099	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SARLGA251_RS10410	2102417..2103619	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SARLGA251_RS10415	2103883..2104020	-	138	WP_000828354.1	hypothetical protein	-
SARLGA251_RS10420	2104148..2104357	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SARLGA251_RS10425	2104369..2104662	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SARLGA251_RS10430	2104828..2106312	+	1485	WP_000571557.1	cardiolipin synthase	-
SARLGA251_RS10435	2106340..2106987	+	648	WP_001187631.1	HD domain-containing protein	-
SARLGA251_RS10440	2107753..2108625	-	873	WP_000725802.1	membrane protein insertase YidC	-
SARLGA251_RS10445	2108711..2109352	-	642	WP_000483148.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T26720 WP_000581792.1 NC_017349:c2104357-2104148 [Staphylococcus aureus subsp. aureus LGA251]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T26720 NC_017349:c2104357-2104148 [Staphylococcus aureus subsp. aureus LGA251]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT26720 WP_000138934.1 NC_017349:c2104662-2104369 [Staphylococcus aureus subsp. aureus LGA251]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT26720 NC_017349:c2104662-2104369 [Staphylococcus aureus subsp. aureus LGA251]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9