Detailed information of TA system TA026719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2084403..2084932
• Replicon: chromosome
• Accession: NC_017349
• Organism: Staphylococcus aureus subsp. aureus LGA251

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SARLGA251_RS10330
• Protein ID: WP_000621175.1
• Coordinates: 2084403..2084765 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SARLGA251_RS10335
• Protein ID: WP_000948331.1
• Coordinates: 2084762..2084932 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SARLGA251_RS10305	2081381..2082151	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SARLGA251_RS10310	2082126..2082605	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SARLGA251_RS10315	2082607..2082933	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SARLGA251_RS10320	2083052..2084053	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SARLGA251_RS10330	2084403..2084765	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SARLGA251_RS10335	2084762..2084932	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SARLGA251_RS10340	2085017..2086165	-	1149	WP_001281160.1	alanine racemase	-
SARLGA251_RS10345	2086231..2086590	-	360	WP_000581198.1	holo-ACP synthase	-
SARLGA251_RS10350	2086594..2087085	-	492	WP_001205919.1	PH domain-containing protein	-
SARLGA251_RS10355	2087072..2088655	-	1584	WP_001294651.1	PH domain-containing protein	-
SARLGA251_RS10360	2088648..2089127	-	480	WP_001287083.1	hypothetical protein	-
SARLGA251_RS10365	2089336..2089896	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T26719 WP_000621175.1 NC_017349:c2084765-2084403 [Staphylococcus aureus subsp. aureus LGA251]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T26719 NC_017349:c2084765-2084403 [Staphylococcus aureus subsp. aureus LGA251]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT26719 WP_000948331.1 NC_017349:c2084932-2084762 [Staphylococcus aureus subsp. aureus LGA251]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT26719 NC_017349:c2084932-2084762 [Staphylococcus aureus subsp. aureus LGA251]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1