Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3390303..3391276 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag PAK35_RS15885 Protein ID WP_003404756.1
Coordinates 3390303..3390803 (-) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag PAK35_RS15890 Protein ID WP_003404754.1
Coordinates 3390800..3391276 (-) Length 159 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS15865 (PAK35_15865) 3386150..3387802 - 1653 WP_003905412.1 IS607 family element RNA-guided endonuclease TnpB -
PAK35_RS15870 (PAK35_15870) 3387802..3388383 - 582 WP_003404760.1 IS607-like element IS1535 family transposase -
PAK35_RS15875 (PAK35_15875) 3388671..3389990 + 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
PAK35_RS15885 (PAK35_15885) 3390303..3390803 - 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
PAK35_RS15890 (PAK35_15890) 3390800..3391276 - 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
PAK35_RS15895 (PAK35_15895) 3391365..3391619 - 255 WP_234708440.1 hypothetical protein -
PAK35_RS15900 (PAK35_15900) 3391619..3393400 - 1782 WP_003404749.1 BCCT family transporter -
PAK35_RS15905 (PAK35_15905) 3393844..3394143 + 300 WP_003404748.1 PE family protein -
PAK35_RS15910 (PAK35_15910) 3394158..3395429 + 1272 WP_003404747.1 PPE family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3345714..3391622 45908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(38-143)

Antitoxin

(74-150)


Sequences


Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T267133 WP_003404756.1 NZ_CP115447:c3390803-3390303 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp


Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT267133 WP_003404754.1 NZ_CP115447:c3391276-3390800 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2


Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References