Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3184026..3184657 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag PAK35_RS14885 Protein ID WP_003405820.1
Coordinates 3184346..3184657 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag PAK35_RS14880 Protein ID WP_003405836.1
Coordinates 3184026..3184346 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS14855 (PAK35_14855) 3179222..3179860 + 639 WP_003898730.1 lipid droplet-associated protein -
PAK35_RS14860 (PAK35_14860) 3179857..3181104 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
PAK35_RS14865 (PAK35_14865) 3181094..3181351 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
PAK35_RS14870 (PAK35_14870) 3181361..3182473 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
PAK35_RS14875 (PAK35_14875) 3182480..3184016 - 1537 Protein_2943 carboxylesterase/lipase family protein -
PAK35_RS14880 (PAK35_14880) 3184026..3184346 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
PAK35_RS14885 (PAK35_14885) 3184346..3184657 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
PAK35_RS14890 (PAK35_14890) 3184769..3185926 + 1158 WP_003898727.1 AI-2E family transporter -
PAK35_RS14895 (PAK35_14895) 3185933..3186577 - 645 WP_003915494.1 DUF4245 domain-containing protein -
PAK35_RS14900 (PAK35_14900) 3186633..3187721 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
PAK35_RS14905 (PAK35_14905) 3187752..3189176 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T267132 WP_003405820.1 NZ_CP115447:3184346-3184657 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT267132 WP_003405836.1 NZ_CP115447:3184026-3184346 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References