Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2004149..2004735 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag PAK35_RS09215 Protein ID WP_003410010.1
Coordinates 2004149..2004493 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag PAK35_RS09220 Protein ID WP_003410014.1
Coordinates 2004487..2004735 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS09180 (PAK35_09180) 1999855..2000454 + 600 WP_003910883.1 L-lysine exporter -
PAK35_RS09185 (PAK35_09185) 2000870..2001298 + 429 WP_003409992.1 cellulose-binding protein -
PAK35_RS09190 (PAK35_09190) 2001524..2002063 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
PAK35_RS09195 (PAK35_09195) 2002583..2003143 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
PAK35_RS09200 (PAK35_09200) 2003140..2003481 - 342 WP_003410003.1 DUF2384 domain-containing protein -
PAK35_RS09205 (PAK35_09205) 2003567..2003824 + 258 WP_003410006.1 hypothetical protein -
PAK35_RS09210 (PAK35_09210) 2003725..2004060 - 336 WP_003410009.1 dehydrogenase -
PAK35_RS09215 (PAK35_09215) 2004149..2004493 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
PAK35_RS09220 (PAK35_09220) 2004487..2004735 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
PAK35_RS09225 (PAK35_09225) 2004835..2007150 - 2316 WP_003899120.1 cation transporter ATPase CptG -
PAK35_RS09230 (PAK35_09230) 2007147..2007419 - 273 WP_003410017.1 DUF1490 family protein -
PAK35_RS09235 (PAK35_09235) 2007472..2007828 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
PAK35_RS09240 (PAK35_09240) 2007985..2008751 + 767 Protein_1832 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T267112 WP_003410010.1 NZ_CP115447:c2004493-2004149 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT267112 WP_003410014.1 NZ_CP115447:c2004735-2004487 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References