Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1961748..1962451 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag PAK35_RS08915 Protein ID WP_003409778.1
Coordinates 1961748..1962077 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag PAK35_RS08920 Protein ID WP_003409780.1
Coordinates 1962074..1962451 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS08895 (PAK35_08895) 1958131..1959201 + 1071 WP_003902252.1 epoxide hydrolase EphB -
PAK35_RS08900 (PAK35_08900) 1959198..1959713 + 516 WP_003409718.1 flavin reductase family protein -
PAK35_RS08905 (PAK35_08905) 1959710..1960771 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
PAK35_RS08910 (PAK35_08910) 1960768..1961538 + 771 WP_003409775.1 SDR family oxidoreductase -
PAK35_RS08915 (PAK35_08915) 1961748..1962077 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PAK35_RS08920 (PAK35_08920) 1962074..1962451 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
PAK35_RS08925 (PAK35_08925) 1962448..1963038 - 591 WP_003409784.1 SEC-C metal-binding domain-containing protein -
PAK35_RS08930 (PAK35_08930) 1963093..1964457 + 1365 WP_003910878.1 HNH endonuclease signature motif containing protein -
PAK35_RS08935 (PAK35_08935) 1964612..1965064 - 453 WP_003899095.1 lipoprotein -
PAK35_RS08940 (PAK35_08940) 1965128..1965529 + 402 WP_003409869.1 hypothetical protein -
PAK35_RS08945 (PAK35_08945) 1965522..1965704 - 183 WP_003409870.1 hypothetical protein -
PAK35_RS08950 (PAK35_08950) 1965818..1966168 - 351 WP_003409871.1 hypothetical protein -
PAK35_RS08955 (PAK35_08955) 1966179..1967081 - 903 WP_003899097.1 hypothetical protein -
PAK35_RS08960 (PAK35_08960) 1967102..1967293 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T267107 WP_003409778.1 NZ_CP115447:c1962077-1961748 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT267107 WP_003409780.1 NZ_CP115447:c1962451-1962074 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References