Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1468768..1469384 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag PAK35_RS06735 Protein ID WP_003407593.1
Coordinates 1469067..1469384 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag PAK35_RS06730 Protein ID WP_003900349.1
Coordinates 1468768..1469070 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS06720 (PAK35_06720) 1464654..1466501 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
PAK35_RS06725 (PAK35_06725) 1466502..1468754 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
PAK35_RS06730 (PAK35_06730) 1468768..1469070 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
PAK35_RS06735 (PAK35_06735) 1469067..1469384 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
PAK35_RS06740 (PAK35_06740) 1469381..1470385 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
PAK35_RS06745 (PAK35_06745) 1470438..1471727 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
PAK35_RS06750 (PAK35_06750) 1471800..1472525 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
PAK35_RS06755 (PAK35_06755) 1472631..1472843 - 213 WP_003898900.1 dodecin family protein -
PAK35_RS06760 (PAK35_06760) 1472904..1473302 + 399 WP_003900351.1 hypothetical protein -
PAK35_RS06765 (PAK35_06765) 1473347..1474375 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T267103 WP_003407593.1 NZ_CP115447:1469067-1469384 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT267103 WP_003900349.1 NZ_CP115447:1468768-1469070 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References