Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 470435..471072 Replicon chromosome
Accession NZ_CP115158
Organism Bacillus amyloliquefaciens strain MBLB 0692

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag O7R04_RS02460 Protein ID WP_003156187.1
Coordinates 470722..471072 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag O7R04_RS02455 Protein ID WP_003156188.1
Coordinates 470435..470716 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O7R04_RS02435 (O7R04_02435) 466800..467399 - 600 WP_139885531.1 rhomboid family intramembrane serine protease -
O7R04_RS02440 (O7R04_02440) 467492..467857 + 366 WP_014304402.1 holo-ACP synthase -
O7R04_RS02445 (O7R04_02445) 468022..469029 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
O7R04_RS02450 (O7R04_02450) 469146..470315 + 1170 WP_003156189.1 alanine racemase -
O7R04_RS02455 (O7R04_02455) 470435..470716 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
O7R04_RS02460 (O7R04_02460) 470722..471072 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
O7R04_RS02465 (O7R04_02465) 471190..472011 + 822 WP_003156182.1 STAS domain-containing protein -
O7R04_RS02470 (O7R04_02470) 472016..472381 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
O7R04_RS02475 (O7R04_02475) 472384..472785 + 402 WP_003156178.1 anti-sigma regulatory factor -
O7R04_RS02480 (O7R04_02480) 472797..473804 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
O7R04_RS02485 (O7R04_02485) 473868..474197 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
O7R04_RS02490 (O7R04_02490) 474194..474676 + 483 WP_003156173.1 anti-sigma B factor RsbW -
O7R04_RS02495 (O7R04_02495) 474642..475430 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
O7R04_RS02500 (O7R04_02500) 475430..476032 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267059 WP_003156187.1 NZ_CP115158:470722-471072 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT267059 WP_003156188.1 NZ_CP115158:470435-470716 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References