Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 32959..33695 Replicon plasmid IncHI1BpNDM-MAR
Accession NZ_CP115151
Organism Enterobacter hormaechei subsp. xiangfangensis strain MDCL 3

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag MUY00_RS23400 Protein ID WP_004098919.1
Coordinates 33213..33695 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag MUY00_RS23395 Protein ID WP_004213599.1
Coordinates 32959..33225 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY00_RS23375 (MUY00_23370) 30472..31476 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
MUY00_RS23380 (MUY00_23375) 31762..32256 + 495 WP_004213594.1 hypothetical protein -
MUY00_RS23385 (MUY00_23380) 32317..32520 + 204 WP_004213596.1 HHA domain-containing protein -
MUY00_RS23390 (MUY00_23385) 32534..32764 + 231 WP_004213598.1 hypothetical protein -
MUY00_RS23395 (MUY00_23390) 32959..33225 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
MUY00_RS23400 (MUY00_23395) 33213..33695 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
MUY00_RS23405 (MUY00_23400) 33896..35299 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
MUY00_RS23410 (MUY00_23405) 35328..35960 - 633 WP_001567369.1 hypothetical protein -
MUY00_RS23415 (MUY00_23410) 36035..37003 + 969 WP_004099053.1 IS5-like element IS903B family transposase -
MUY00_RS23420 (MUY00_23415) 37465..38692 + 1228 Protein_39 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid rmtC / blaNDM-1 / sul1 / aac(3)-IId / qacE / aac(6')-Ib iucA / iucB / iucC / iucD / iutA / rmpA 1..200030 200030
- inside IScluster/Tn rmtC / blaNDM-1 / sul1 / aac(3)-IId - 16..38692 38676


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T267056 WP_004098919.1 NZ_CP115151:33213-33695 [Enterobacter hormaechei subsp. xiangfangensis]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT267056 WP_004213599.1 NZ_CP115151:32959-33225 [Enterobacter hormaechei subsp. xiangfangensis]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References