Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2725117..2725753 Replicon chromosome
Accession NZ_CP114899
Organism Bacillus subtilis strain

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag O6U12_RS14645 Protein ID WP_003156187.1
Coordinates 2725117..2725467 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag O6U12_RS14650 Protein ID WP_003225183.1
Coordinates 2725472..2725753 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6U12_RS14605 (O6U12_14605) 2720171..2720770 - 600 WP_017696999.1 phosphoserine phosphatase RsbX -
O6U12_RS14610 (O6U12_14610) 2720770..2721558 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
O6U12_RS14615 (O6U12_14615) 2721524..2722006 - 483 WP_003234299.1 anti-sigma B factor RsbW -
O6U12_RS14620 (O6U12_14620) 2722003..2722332 - 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
O6U12_RS14625 (O6U12_14625) 2722394..2723401 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
O6U12_RS14630 (O6U12_14630) 2723413..2723814 - 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
O6U12_RS14635 (O6U12_14635) 2723818..2724183 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
O6U12_RS14640 (O6U12_14640) 2724188..2725012 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
O6U12_RS14645 (O6U12_14645) 2725117..2725467 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
O6U12_RS14650 (O6U12_14650) 2725472..2725753 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
O6U12_RS14655 (O6U12_14655) 2725869..2727038 - 1170 WP_014478898.1 alanine racemase -
O6U12_RS14660 (O6U12_14660) 2727153..2728169 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
O6U12_RS14665 (O6U12_14665) 2728335..2728700 - 366 WP_014478897.1 holo-ACP synthase -
O6U12_RS14670 (O6U12_14670) 2728795..2729394 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T267043 WP_003156187.1 NZ_CP114899:c2725467-2725117 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT267043 WP_003225183.1 NZ_CP114899:c2725753-2725472 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References