266988

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4192081..4192760 Replicon chromosome
Accession NZ_CP114894
Organism Escherichia coli strain CM19

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag O6X69_RS20490 Protein ID WP_000854672.1
Coordinates 4192081..4192422 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag O6X69_RS20495 Protein ID WP_000070395.1
Coordinates 4192443..4192760 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6X69_RS20465 (4187358) 4187358..4187759 + 402 WP_000174689.1 sigma factor-binding protein Crl -
O6X69_RS20470 (4187798) 4187798..4188853 - 1056 WP_000749863.1 phosphoporin PhoE -
O6X69_RS20475 (4189141) 4189141..4190244 + 1104 WP_001285288.1 glutamate 5-kinase -
O6X69_RS20480 (4190256) 4190256..4191509 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
O6X69_RS20490 (4192081) 4192081..4192422 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
O6X69_RS20495 (4192443) 4192443..4192760 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
O6X69_RS20500 (4192779) 4192779..4193000 - 222 WP_000691994.1 DUF987 domain-containing protein -
O6X69_RS20505 (4193009) 4193009..4193485 - 477 WP_000811693.1 RadC family protein -
O6X69_RS20510 (4193501) 4193501..4193959 - 459 WP_000211838.1 antirestriction protein -
O6X69_RS20515 (4194057) 4194057..4194296 - 240 WP_000194654.1 DUF905 family protein -
O6X69_RS20520 (4194373) 4194373..4194840 - 468 WP_001547765.1 protein YkfB -
O6X69_RS20525 (4194863) 4194863..4195306 - 444 WP_269707801.1 lipoprotein YafY -
O6X69_RS20530 (4195306) 4195306..4195533 - 228 WP_001548158.1 protein YpjK -
O6X69_RS20535 (4195529) 4195529..4195720 - 192 Protein_3986 DeoR family transcriptional regulator -
O6X69_RS20540 (4195937) 4195937..4196758 - 822 WP_000197389.1 DUF932 domain-containing protein -
O6X69_RS20545 (4196850) 4196850..4197713 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T266988 WP_000854672.1 NZ_CP114894:c4192422-4192081 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT266988 WP_000070395.1 NZ_CP114894:c4192760-4192443 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References