266976

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2118335..2119772 Replicon chromosome
Accession NZ_CP114894
Organism Escherichia coli strain CM19

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag O6X69_RS10445 Protein ID WP_000155570.1
Coordinates 2118335..2119408 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag O6X69_RS10450 Protein ID WP_000461704.1
Coordinates 2119401..2119772 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6X69_RS10430 (2113768) 2113768..2115957 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
O6X69_RS10435 (2115954) 2115954..2117570 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
O6X69_RS10440 (2117930) 2117930..2118193 - 264 WP_000502050.1 DUF1524 domain-containing protein -
O6X69_RS10445 (2118335) 2118335..2119408 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
O6X69_RS10450 (2119401) 2119401..2119772 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
O6X69_RS10455 (2120127) 2120127..2120990 + 864 WP_000787597.1 GTPase family protein -
O6X69_RS10460 (2121082) 2121082..2121903 + 822 WP_000197391.1 DUF932 domain-containing protein -
O6X69_RS10465 (2122120) 2122120..2122821 + 702 WP_000189409.1 WYL domain-containing protein -
O6X69_RS10470 (2122862) 2122862..2123098 + 237 WP_001144029.1 protein YpjK -
O6X69_RS10475 (2123098) 2123098..2123541 + 444 WP_000824222.1 lipoprotein YfjS -
O6X69_RS10480 (2123565) 2123565..2124032 + 468 WP_000702495.1 protein YfjT -
O6X69_RS10485 (2124419) 2124419..2124571 - 153 Protein_2044 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2108576..2155009 46433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T266976 WP_000155570.1 NZ_CP114894:2118335-2119408 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT266976 WP_000461704.1 NZ_CP114894:2119401-2119772 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References